General Stability | Organism |
---|---|
thermostable up to 90°C, only in the presence of salts. Complete stability in presence of 0.4-0.5 M K2HPO4 | Archaeoglobus fulgidus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | 0.15M-0.2M (NH4)2SO4 stimulates by a factor of 1.3. Higher concentrations are inhibitory | Archaeoglobus fulgidus | |
K2HPO4 | 0.15M-0.2M K2HPO4 stimulates by a factor of 1.3. Higher concentrations are inhibitory | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
coenzyme F420 | - |
Archaeoglobus fulgidus | |
0.017 | - |
5,10-methylenetetrahydromethanopterin | - |
Archaeoglobus fulgidus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | 0.15M-0.2M (NH4)2SO4 stimulates by a factor of 1.3. Higher concentrations are inhibitory | Archaeoglobus fulgidus | |
K+ | 0.15M-0.2M K2HPO4 stimulates by a factor of 1.3. Higher concentrations are inhibitory | Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, SDS-PAGE | Archaeoglobus fulgidus |
140000 | - |
gel filtration | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1560 | - |
- |
Archaeoglobus fulgidus |
Storage Stability | Organism |
---|---|
-20°C, stable for several weeks | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | r | Archaeoglobus fulgidus | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
? | |
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | r | Archaeoglobus fulgidus VC-19 / DSM 4304 | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Archaeoglobus fulgidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
thermostable up to 90°C, only in the presence of salts. Complete stability in presence of 0.4-0.5 M K2HPO4 | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Archaeoglobus fulgidus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme F420 | required | Archaeoglobus fulgidus |